| MWexperimental | 105 | kDa |
| MWexpected | 104 | kDa |
| VPorod | 176 | nm3 |
|
log I(s)
5.41×101
5.41×100
5.41×10-1
5.41×10-2
|
s, nm-1
|
1-deoxy-D-xylulose 5-phosphate reductoisomerase (DXR) as target for anti Toxoplasma gondii agents: crystal structure, biochemical characterisation and biological evaluation of inhibitors
Mazzone F, Hoeppner A, Reiners J, Gertzen C, Applegate V, Abdullaziz M, Gottstein J, Degrandi D, Wesemann M, Kurz T, Smits S, Pfeffer K, Biochemical Journal (2024) DOISASDS47 – 1-Deoxy-D-xylulose-5-phosphate reductoisomerase (DXR)
1-deoxy-D-xylulose-5-phosphate reductoisomerase (G328C, K361E, S551G)|
|
|
Fits and models
|
Rg, nm
|
|
Synchrotron SAXS data from solutions of 1-Deoxy-D-xylulose-5-phosphate reductoisomerase in 20 mM Tris/HCl, 150 mM NaCl, 40 mM MgCl2, 2% glycerol,, pH 7.5 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus3 2M detector at a sample-detector distance of 2.8 m and at a wavelength of λ = 0.099 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 300.00 μl sample at 8 mg/ml was injected at a 0.50 ml/min flow rate onto a GE Superdex 200 Increase 10/300 column at 10°C. 1500 successive 2 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
|
|
|||||||||||||||||||||||||||