Type 1 secretion necessitates a tight interplay between all domains of the ABC transporter

Anlauf M, Bilsing F, Reiners J, Spitz O, Hachani E, Smits S, Schmitt L, Scientific Reports 14(1) (2024) DOI

SASDS56 – Nucleotide binding domain, NBD (ABC transporter domain) from the RTX toxin ABC transporter RtxB

RTX toxin transporter
MWexperimental 30 kDa
MWexpected 28 kDa
VPorod 48 nm3
log I(s) 2.02×10-2 2.02×10-3 2.02×10-4 2.02×10-5
RTX toxin transporter small angle scattering data  s, nm-1
ln I(s)
RTX toxin transporter Guinier plot ln 2.03×10-2 Rg: 2.2 nm 0 (2.2 nm)-2 s2
(sRg)2I(s)/I(0)
RTX toxin transporter Kratky plot 1.104 0 √3 sRg
p(r)
RTX toxin transporter pair distance distribution function Rg: 2.2 nm 0 Dmax: 7.6 nm

Data validation

Fits and models

log I(s)
 s, nm-1
RTX toxin transporter GASBOR model
Synchrotron SAXS data from solutions of the nucleotide binding domain from RtxB in 100 mM HEPES, 10 % glycerol, pH 8 were collected on the EMBL P12 beam line at PETRA III, (DESY; Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 1.93 mg/ml was measured at 10°C. 40 successive 0.095 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

RTX toxin transporter (RtxB-NBD)
Mol. type   Protein
Organism   Kingella kingae ATCC 23330
Olig. state   Monomer
Mon. MW   27.7 kDa
 
UniProt   F5S9L7 (468-708)
Sequence   FASTA