Domain crossover in the reductase subunit of NADPH-dependent assimilatory sulfite reductase.

Walia N, Murray DT, Garg Y, He H, Weiss KL, Nagy G, Elizabeth Stroupe M, J Struct Biol 215(4):108028 (2023) Europe PMC

SASDS72 – Sulfite reductase truncated linker (ΔAAPSQS) dodecamer

Sulfite reductase [NADPH] hemoprotein beta-component
Sulfite reductase [NADPH] flavoprotein alpha-component (Δ212-217)
MWexperimental 873 kDa
MWexpected 784 kDa
VPorod 1260 nm3
log I(s) 3.00×100 3.00×10-1 3.00×10-2 3.00×10-3
Sulfite reductase [NADPH] hemoprotein beta-component Sulfite reductase [NADPH] flavoprotein alpha-component (Δ212-217) small angle scattering data  s, nm-1
ln I(s)
Sulfite reductase [NADPH] hemoprotein beta-component Sulfite reductase [NADPH] flavoprotein alpha-component (Δ212-217) Guinier plot ln 3.00×100 Rg: 10.8 nm 0 (10.8 nm)-2 s2
(sRg)2I(s)/I(0)
Sulfite reductase [NADPH] hemoprotein beta-component Sulfite reductase [NADPH] flavoprotein alpha-component (Δ212-217) Kratky plot 1.104 0 √3 sRg
p(r)
Sulfite reductase [NADPH] hemoprotein beta-component Sulfite reductase [NADPH] flavoprotein alpha-component (Δ212-217) pair distance distribution function Rg: 10 nm 0 Dmax: 30.3 nm

Data validation

There are no models related to this curve.

SANS data from solutions of sulfite reductase truncated linker dodecamer in 50 mM KPi, 100 mM NaCl, 1 mM EDTA, pH 7.8 were collected using the EQ-SANS (BL-6) spallation neutron source (Oak Ridge, Tennessee, USA). This sample is a dodecamer of His-tagged sulfite reductase flavoprotein truncated linker octamer (6 amino acid deletion in linker region) and four copies of hemoprotein. Three instrument configurations were used: 9 m sample-to-detector distance with 1.5 nm wavelength band, 4 m sample-to-detector distance with 0.6 nm wavelength band, and 1.3 m sample-to-detector distance with 0.4 nm wavelength band. Sample concentration: 3 mg/mL. Neutron exposure time: 3 hours / instrument configuration. Sample temperature: 8°C.

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Sulfite reductase [NADPH] hemoprotein beta-component
Mol. type   Protein
Organism   Escherichia coli (strain K12)
Olig. state   Tetramer
Mon. MW   64.0 kDa
 
UniProt   P17846 (1-570)
Sequence   FASTA
 
Sulfite reductase [NADPH] flavoprotein alpha-component (Δ212-217)
Mol. type   Protein
Organism   Escherichia coli (strain K12)
Olig. state   Octamer
Mon. MW   66 kDa
 
UniProt   P38038 (1-599)
Sequence   FASTA