| MWexperimental | 95 | kDa |
| MWexpected | 96 | kDa |
| VPorod | 190 | nm3 |
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log I(s)
5.12×10-2
5.12×10-3
5.12×10-4
5.12×10-5
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s, nm-1
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Calcium modulates the tethering of BCOR-PRC1.1 enzymatic core to KDM2B via liquid-liquid phase separation.
Chen R, Shen F, Zhang Y, Sun M, Dong Y, Yin Y, Su C, Peng C, Liu J, Xu J, Commun Biol 7(1):1112 (2024) Europe PMCSASDS77 – Complex of BCL-6 corepressor ANK-linker-PUFD bound to Polycomb group RING finger protein 1 RAWUL, Lysine-specific demethylase 2B F-box-LRRs, and S-phase kinase-associated protein 1
Lysine-specific demethylase 2BPolycomb group RING finger protein 1
S-phase kinase-associated protein 1
BCL-6 corepressor
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Fits and models
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Synchrotron SAXS data from solutions of the complex BCOR(ANK-linker-PUFD)/PCGF1(RAWUL)/KDM2B(F-box-LRRs)/SKP1 in 20 mM Tris-HCl, 150 mM NaCl, pH 7.5 were collected on the BL19U2 beam line at the Shanghai Synchrotron Radiation Facility (SSRF; Shanghai, China) using a Pilatus 1M detector at a sample-detector distance of 2.2 m and at a wavelength of λ = 0.1033 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 100.00 μl sample at 10 mg/ml was injected at a 0.50 ml/min flow rate onto a column at 25°C. 1798 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
SEC column = UNKNOWN |
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