Synchrotron SAXS data from solutions of Long SARS-CoV-2 Non-structural protein 10 in 10 mM HEPES, 300 mM NaCl, 1.5% (v/v) glycerol, 2 mM DTT, pH 7.5 were collected on the B21 beam line at the Diamond Light Source (Didcot, UK) using a Eiger 4M detector at a sample-detector distance of 3.7 m and at a wavelength of λ = 0.09464 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 35.00 μl sample at 10 mg/ml was injected at a 0.08 ml/min flow rate onto a Cytiva Superdex 75 10/300 column at 4°C. 21 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
Non-structural protein 10 from SARS-CoV-2 with a C-terminal truncation.
s, nm-1
