Domain crossover in the reductase subunit of NADPH-dependent assimilatory sulfite reductase.

Walia N, Murray DT, Garg Y, He H, Weiss KL, Nagy G, Elizabeth Stroupe M, J Struct Biol 215(4):108028 (2023) Europe PMC

SASDS82 – Reconstituted sulfite reductase crosslinked dodecamer with partially deuterated hemoprotein in 0% D2O

Sulfite reductase [NADPH] flavoprotein alpha-component (E121C, C162T, C552S, N556C)
Sulfite reductase [NADPH] hemoprotein beta-component
MWexperimental 873 kDa
MWexpected 822 kDa
VPorod 1010 nm3
log I(s) 6.20×100 6.20×10-1 6.20×10-2 6.20×10-3
Sulfite reductase [NADPH] flavoprotein alpha-component (E121C, C162T, C552S, N556C) Sulfite reductase [NADPH] hemoprotein beta-component small angle scattering data  s, nm-1
ln I(s)
Sulfite reductase [NADPH] flavoprotein alpha-component (E121C, C162T, C552S, N556C) Sulfite reductase [NADPH] hemoprotein beta-component Guinier plot ln 6.21×100 Rg: 9.5 nm 0 (9.5 nm)-2 s2
(sRg)2I(s)/I(0)
Sulfite reductase [NADPH] flavoprotein alpha-component (E121C, C162T, C552S, N556C) Sulfite reductase [NADPH] hemoprotein beta-component Kratky plot 1.104 0 √3 sRg
p(r)
Sulfite reductase [NADPH] flavoprotein alpha-component (E121C, C162T, C552S, N556C) Sulfite reductase [NADPH] hemoprotein beta-component pair distance distribution function Rg: 9.8 nm 0 Dmax: 28.3 nm

Data validation

There are no models related to this curve.

SANS data from solutions of reconstituted sulfite reductase crosslinked dodecamer with partially deuterated hemoprotein in 0% D2O, in 50 mM KPi, 100 mM NaCl, 1 mM EDTA, pH 7.8 were collected using the EQ-SANS (BL-6) spallation neutron source (Oak Ridge, Tennessee, USA). This sample is a dodecamer of His-tagged sulfite reductase flavoprotein crosslinked octamer and four copies of partially deuterated hemoprotein. Three instrument configurations were used: 9 m sample-to-detector distance with 1.5 nm wavelength band, 4 m sample-to-detector distance with 0.6 nm wavelength band, and 1.3 m sample-to-detector distance with 0.4 nm wavelength band. Sample concentration: 7 mg/mL. Neutron exposure time: 3 hours / instrument configuration. Sample temperature: 8°C.

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Sulfite reductase [NADPH] flavoprotein alpha-component (E121C, C162T, C552S, N556C)
Mol. type   Protein
Organism   Escherichia coli (strain K12)
Olig. state   Octamer
Mon. MW   70.7 kDa
 
UniProt   P38038 (1-599)
Sequence   FASTA
 
Sulfite reductase [NADPH] hemoprotein beta-component
Mol. type   Protein
Organism   Escherichia coli (strain K12)
Olig. state   Tetramer
Mon. MW   64 kDa
 
UniProt   P17846 (1-570)
Sequence   FASTA