| MWexperimental | 180 | kDa |
| MWexpected | 181 | kDa |
| VPorod | 300 | nm3 |
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log I(s)
6.41×102
6.41×101
6.41×100
6.41×10-1
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s, nm-1
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Flexibility and distributive synthesis regulate RNA priming and handoff in human DNA polymerase α-primase
Cordoba J, Mullins E, Salay L, Eichman B, Chazin W, Journal of Molecular Biology :168330 (2023) DOISASDS87 – polymerase alpha-primase polΔcat-prim in the absence of substrate
DNA polymerase alpha subunit BDNA polymerase alpha catalytic subunit
DNA primase large subunit
DNA primase small subunit
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Fits and models
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Synchrotron SAXS data from solutions of polΔcat-prim in the absence of substrate in 20 mM HEPES, 150 mM NaCl, 5 mM MgCl2, 1 mM TCEP, pH 7.5 were collected on the 12.3.1 (SIBYLS) beam line at the Advanced Light Source (ALS; Berkeley, CA, USA) using a Pilatus3 X 2M detector at a sample-detector distance of 1.5 m and at a wavelength of λ = 0.103 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 60.00 μl sample at 5 mg/ml was injected at a 0.65 ml/min flow rate onto a Shodex LW-803 column at 23°C. 500 successive 3 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
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