Domain crossover in the reductase subunit of NADPH-dependent assimilatory sulfite reductase.

Walia N, Murray DT, Garg Y, He H, Weiss KL, Nagy G, Elizabeth Stroupe M, J Struct Biol 215(4):108028 (2023) Europe PMC

SASDS92 – Reconstituted sulfite reductase crosslinked dodecamer with partially deuterated hemoprotein in 100% D2O

Sulfite reductase [NADPH] hemoprotein beta-component
Sulfite reductase [NADPH] flavoprotein alpha-component (E121C, C162T, C552S, N556C)
MWexperimental 715 kDa
MWexpected 822 kDa
VPorod 839 nm3
log I(s) 4.71×100 4.71×10-1 4.71×10-2 4.71×10-3
Sulfite reductase [NADPH] hemoprotein beta-component Sulfite reductase [NADPH] flavoprotein alpha-component (E121C, C162T, C552S, N556C) small angle scattering data  s, nm-1
ln I(s)
Sulfite reductase [NADPH] hemoprotein beta-component Sulfite reductase [NADPH] flavoprotein alpha-component (E121C, C162T, C552S, N556C) Guinier plot ln 4.72×100 Rg: 8.2 nm 0 (8.2 nm)-2 s2
(sRg)2I(s)/I(0)
Sulfite reductase [NADPH] hemoprotein beta-component Sulfite reductase [NADPH] flavoprotein alpha-component (E121C, C162T, C552S, N556C) Kratky plot 1.104 0 √3 sRg
p(r)
Sulfite reductase [NADPH] hemoprotein beta-component Sulfite reductase [NADPH] flavoprotein alpha-component (E121C, C162T, C552S, N556C) pair distance distribution function Rg: 8.3 nm 0 Dmax: 25.1 nm

Data validation

There are no models related to this curve.

SANS data from solutions of reconstituted sulfite reductase xlinked dodecamer with partially deuterated hemoprotein in 100% D2O, 50 mM KPi, 100 mM NaCl, 1 mM EDTA, pH 7.8 were collected using the EQ-SANS (BL-6) spallation neutron source (Oak Ridge, Tennessee, USA). This sample is a dodecamer of His-tagged sulfite reductase flavoprotein crosslinked octamer and four copies of partially deuterated hemoprotein.Three instrument configurations were used: 9 m sample-to-detector distance with 1.5 nm wavelength band, 4 m sample-to-detector distance with 0.6 nm wavelength band, and 1.3 m sample-to-detector distance with 0.4 nm wavelength band. Sample concentration: 7 mg/mL. Neutron exposure time: 3 hours / instrument configuration. Sample temperature: 8°C

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Sulfite reductase [NADPH] hemoprotein beta-component
Mol. type   Protein
Organism   Escherichia coli (strain K12)
Olig. state   Tetramer
Mon. MW   64.0 kDa
 
UniProt   P17846 (1-570)
Sequence   FASTA
 
Sulfite reductase [NADPH] flavoprotein alpha-component (E121C, C162T, C552S, N556C)
Mol. type   Protein
Organism   Escherichia coli (strain K12)
Olig. state   Octamer
Mon. MW   70.7 kDa
 
UniProt   P38038 (1-599)
Sequence   FASTA