SASDSJ9 – SAXS data of trypsinized Clostridium perfringens enterotoxin

Heat-labile enterotoxin B chain

MW^experimental	30	kDa
MW^expected	33	kDa
V^Porod	40	nm³

log I(s) 5.90×10^-3 5.90×10^-4 5.90×10^-5 5.90×10^-6

Heat-labile enterotoxin B chain small angle scattering data

s, nm^-1

Log plot Log-Log plot

ln I(s)

Heat-labile enterotoxin B chain Guinier plot

ln 5.91×10^-3 R_g: 2.6 nm 0 (2.6 nm)^-2 s²

(sR_g)²I(s)/I(0)

Heat-labile enterotoxin B chain Kratky plot

1.104 0 √3 sR_g

p(r)	R_g: 2.7 nm 0 D_max: 9.5 nm

Data validation

Experimental data range

p(r) fit to data

Metric

Value

s_min D_max / π

0.3

N_Shannon

Worse

Better

Metric

Value

p_cormap

χ²

0.015
0.954

Worse

Better

Fits and models

log I(s)	s, nm^-1
+3 Δ/σ -3	s, nm^-1

Heat-labile enterotoxin B chain GASBOR model

log I(s)	s, nm^-1
+3 Δ/σ -3	s, nm^-1

Heat-labile enterotoxin B chain PDB (PROTEIN DATA BANK) model

Synchrotron SAXS data from solutions of trypsinized Clostridium perfringens enterotoxin in 10 mM HEPES, 100 mM NaCl, 2% glycerol, pH 7.4 were collected on the BioCAT 18ID beam line at the Advanced Photon Source (APS), Argonne National Laboratory (Lemont, IL, USA) using a Pilatus3 X 1M detector at a sample-detector distance of 3.6 m and at a wavelength of λ = 0.1033 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 250.00 μl sample at 7 mg/ml was injected at a 0.60 ml/min flow rate onto a Cytiva Superdex 200 Increase 10/300 column at 22°C. 1360 successive 0.500 second frames were collected through the entire SEC elution. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Heat-labile enterotoxin B chain (CpE)
Mol. type		Protein
Organism		Clostridium perfringens
Olig. state		Monomer
Mon. MW		33.0 kDa

UniProt		P01558 (26-319)
Sequence		FASTA

PDB ID		2XH6