Synchrotron SAXS data from solutions of full-length HSF2BP in 25 mM Tris-HCl pH 7.5, 250 mM NaCl, 5 mM β-mercaptoethanol were collected on the SWING beam line at SOLEIL (Saint-Aubin, France) using a Eiger 4M detector at a sample-detector distance of 2 m and at a wavelength of λ = 0.103 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 50.00 μl sample at 6 mg/ml was injected at a 0.20 ml/min flow rate onto a Agilent Bio SEC-3, 100 Å column at 293.2°C. 36 successive 0.990 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
s, nm-1
