SAXS data from solutions of A and C rich mixed sequence single stranded RNA in 100 mM NaCl, 10 mM MOPS, 20 µM EDTA, pH 7 were collected using a Xenocs BioXolver L with GeniX3D instrument (University of Copenhagen, Department of Drug Design and Pharmacology) equipped with a Pilatus3 R 300K detector at a sample-detector distance of 0.7 m and at a wavelength of λ = 0.154189 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 1 and 2.9 mg/ml were measured at 25°C. 10 successive 120 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentrations were extrapolated to infinite dilution and merged with the higher concentration data to yield the final composite scattering curve.
s, nm-1
