| MWexperimental | 59 | kDa |
| MWexpected | 54 | kDa |
| VPorod | 69 | nm3 |
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log I(s)
6.09×10-2
6.09×10-3
6.09×10-4
6.09×10-5
|
s, nm-1
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Integrating molecular dynamics simulation with small- and wide-angle X-ray scattering to unravel the flexibility, antigen-blocking, and protease-restoring functions in a hindrance-based pro-antibody.
Liao JM, Hong ST, Wang YT, Cheng YA, Ho KW, Toh SI, Shih O, Jeng US, Lyu PC, Hu IC, Huang MY, Chang CY, Cheng TL, Protein Sci 33(9):e5124 (2024) Europe PMCSASDSN8 – Pro-Nivolumab, Lu02
Pro-Nivolumab, Lu02|
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Fits and models
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Synchrotron SAXS
data from solutions of
Pro-Nivolumab, Lu02
in
20 mM Tris, 100 mM NaCl, pH 8
were collected
on the
TPS13A beam line
at the NSRRC storage ring
(Hsinchu, Taiwan)
using a Eiger X 1M & 9M detector
at a sample-detector distance of 2.5 m and
at a wavelength of λ = 0.16 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 100.00 μl sample
at 10.6 mg/ml was injected at a 0.35 ml/min flow rate
onto a Agilent Bio SEC-3, 300 Å column
at 10°C.
Five successive
2 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
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