| MWexperimental | 56 | kDa |
| MWexpected | 48 | kDa |
| VPorod | 91 | nm3 |
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log I(s)
6.41×10-2
6.41×10-3
6.41×10-4
6.41×10-5
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s, nm-1
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14-3-3 protein inhibits CaMKK1 by blocking the kinase active site with its last two C-terminal helices.
Petrvalska O, Honzejkova K, Koupilova N, Herman P, Obsilova V, Obsil T, Protein Sci :e4805 (2023) Europe PMCSASDSQ7 – Calcium/calmodulin-dependent protein kinase kinase 2 (CaMKK2)
Calcium/calmodulin-dependent protein kinase kinase 2|
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Fits and models
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Synchrotron SAXS data from solutions of CaMKK2 in 50 mM Tris-HCl, 150 mM NaCl, 1 mM TCEP, 3% (w/v) glycerol, pH 7.5 were collected on the EMBL P12 beam line at PETRA III (DESY; Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 50.00 μl sample at 9.4 mg/ml was injected at a 0.30 ml/min flow rate onto a GE Superdex 200 Increase 5/150 column at 20°C. 1320 successive 0.495 second frames were collected through the entire SEC elution; 27 SEC sample peak frames were selected for processing to generate the profile displayed in this entry. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
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