SARS-CoV-2 N-protein variants: N1-246 and IDL176-246

Guillem Hernandez.

SASDSR9 – SARS-CoV-2 N-protein (N1-245; residues 1-245): 38.2 µM

Nucleoprotein
MWI(0) 51 kDa
MWexpected 27 kDa
VPorod 56 nm3
log I(s) 5.39×10-2 5.39×10-3 5.39×10-4 5.39×10-5
Nucleoprotein small angle scattering data  s, nm-1
ln I(s)
Nucleoprotein Guinier plot ln 5.40×10-2 Rg: 3.3 nm 0 (3.3 nm)-2 s2
(sRg)2I(s)/I(0)
Nucleoprotein Kratky plot 1.104 0 3 sRg
p(r)
Nucleoprotein pair distance distribution function Rg: 3.6 nm 0 Dmax: 15.5 nm

Data validation


There are no models related to this curve.

Synchrotron SAXS data from solutions of SARS-CoV-2 N-protein (amino acids 1-245) in 100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH 8 were collected on the B21 beam line at the Diamond Light Source (Didcot, UK) using a Eiger 4M detector at a sample-detector distance of 3.7 m and at a wavelength of λ = 0.0954 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 1.00 mg/ml was measured at 10°C. 10 successive 2 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Nucleoprotein (Nprotein1-245)
Mol. type   Protein
Organism   Severe acute respiratory syndrome coronavirus 2
Olig. state   Monomer
Mon. MW   27.4 kDa
 
UniProt   P0DTC9 (1-245)
Sequence   FASTA