Evolutionary conservation of the structure and function of meiotic Rec114-Mei4 and Mer2 complexes.

Daccache D, De Jonge E, Liloku P, Mechleb K, Haddad M, Corthaut S, Sterckx YG, Volkov AN, Claeys Bouuaert C, Genes Dev 37(11-12):535-553 (2023) Europe PMC

SASDSS2 – Arabidopsis thaliana PRD3 coiled-coiled with N-terminal SUMO-tag

Putative recombination initiation defects 3
MWexperimental 136 kDa
MWexpected 129 kDa
log I(s) 1.31×10-2 1.31×10-3 1.31×10-4 1.31×10-5
Putative recombination initiation defects 3 small angle scattering data  s, nm-1
ln I(s)
Putative recombination initiation defects 3 Guinier plot ln 1.31×10-2 Rg: 7.8 nm 0 (7.8 nm)-2 s2
(sRg)2I(s)/I(0)
Putative recombination initiation defects 3 Kratky plot 1.104 0 √3 sRg
p(r)
Putative recombination initiation defects 3 pair distance distribution function Rg: 8.2 nm 0 Dmax: 27.5 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Putative recombination initiation defects 3 OTHER model
Putative recombination initiation defects 3 OTHER model
Putative recombination initiation defects 3 OTHER model
Putative recombination initiation defects 3 OTHER model
Putative recombination initiation defects 3 OTHER model
Putative recombination initiation defects 3 OTHER model
Putative recombination initiation defects 3 OTHER model
Putative recombination initiation defects 3 OTHER model
Putative recombination initiation defects 3 OTHER model
Putative recombination initiation defects 3 OTHER model
log I(s)
 s, nm-1
Synchrotron SAXS data from solutions of Arabidopsis thaliana PRD3 coiled-coiled with N-terminal SUMO-tag in 25 mM HEPES-NaOH, 500 mM NaCl, 5 mM EDTA, 5% glycerol, 1 mM DTT, pH 7.5 were collected on the SWING beam line at the SOLEIL storage ring (Saint-Aubin, France) using a Eiger 4M detector at a sample-detector distance of 2 m and at a wavelength of λ = 0.099 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 90.00 μl sample at 9 mg/ml was injected at a 0.20 ml/min flow rate onto a Shodex KW404-4F column at 15°C. 960 successive 0.990 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Putative recombination initiation defects 3 (PRD3)
Mol. type   Protein
Organism   Arabidopsis thaliana
Olig. state   Tetramer
Mon. MW   32.2 kDa
 
UniProt   Q0WWX5 (126-269)
Sequence   FASTA