SARS-CoV-2 N-protein variants: N1-246 and IDL176-246

Guillem Hernandez.

SASDSW9 – SARS-CoV-2 N-protein (N1-245, residues 1-245) L223P, L227P and L230P triple mutant: 72.7 µM

Nucleoprotein (L223P, L227P, L230P)
MWI(0) 38 kDa
MWexpected 27 kDa
VPorod 47 nm3
log I(s) 4.67×101 4.67×100 4.67×10-1 4.67×10-2
Nucleoprotein (L223P, L227P, L230P) small angle scattering data  s, nm-1
ln I(s)
Nucleoprotein (L223P, L227P, L230P) Guinier plot ln 4.68×101 Rg: 3.5 nm 0 (3.5 nm)-2 s2
(sRg)2I(s)/I(0)
Nucleoprotein (L223P, L227P, L230P) Kratky plot 1.104 0 3 sRg
p(r)
Nucleoprotein (L223P, L227P, L230P) pair distance distribution function Rg: 3.7 nm 0 Dmax: 14.5 nm

Data validation


There are no models related to this curve.

Synchrotron SAXS data from solutions of SARS-CoV-2 N-protein triple mutant (amino acids 1-245; L223P, L227P and L230P) in 100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH 8 were collected on the B21 beam line at the Diamond Light Source (Didcot, UK) using a Eiger 4M detector at a sample-detector distance of 2.8 m and at a wavelength of λ = 0.0954 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 2.00 mg/ml was measured at 10°C. 10 successive 2 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Nucleoprotein (L223P, L227P, L230P) (N1-246 L2P)
Mol. type   Protein
Organism   Severe acute respiratory syndrome coronavirus 2
Olig. state   Monomer
Mon. MW   27.3 kDa
 
UniProt   P0DTC9 (1-245)
Sequence   FASTA