Synchrotron SAXS data from solutions of the DNA binding domain of hepatocyte nuclear factor 1-alpha in 20 mM HEPES, 500 mM NaCl, 1 mM TCEP, pH 8 were collected on the EMBL P12 beam line at PETRA III (DESY; Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 2.60 mg/ml was measured at 20°C. 39 successive 0.045 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
Bovine serum albumin was used as a standard protein for molecular weight determination. A dilution series (0.5 mg/ml–2.6 mg/ml) was prepared in order to investigate potential concentration-dependent effects.
s, nm-1
