SARS-CoV-2 N-protein variants: N1-246 and IDL176-246

Guillem Hernandez.

SASDSZ9 – SARS-CoV-2 N-protein InterDomain Linker (IDL, residues 176-245): 238 µM

Nucleoprotein
MWI(0) 9 kDa
MWexpected 8 kDa
VPorod 19 nm3
log I(s) 2.65×101 2.65×100 2.65×10-1 2.65×10-2
Nucleoprotein small angle scattering data  s, nm-1
ln I(s)
Nucleoprotein Guinier plot ln 2.65×101 Rg: 3.2 nm 0 (3.2 nm)-2 s2
(sRg)2I(s)/I(0)
Nucleoprotein Kratky plot 1.104 0 3 sRg
p(r)
Nucleoprotein pair distance distribution function Rg: 3.2 nm 0 Dmax: 14 nm

Data validation


There are no models related to this curve.

Synchrotron SAXS data from solutions of the SARS-CoV-2 N-protein inter-domain linker (IDL, amino acids 176-245) in 100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH 8 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.8 m and at a wavelength of λ = 0.0999 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 2.00 mg/ml was measured at 10°C. 10 successive 2 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Nucleoprotein (Nprotein-IDL)
Mol. type   Protein
Organism   Severe acute respiratory syndrome coronavirus 2
Olig. state   Monomer
Mon. MW   8.4 kDa
 
UniProt   P0DTC9 (176-245)
Sequence   FASTA