From isolated polyelectrolytes to star-like assemblies: the role of sequence heterogeneity on the statistical structure of the intrinsically disordered neurofilament-low tail domain.

Kravikass M, Koren G, Saleh OA, Beck R, Eur Phys J E Soft Matter 47(2):13 (2024) Europe PMC

SASDT57 – Truncated Neurofilament low intrinsically disordered tail domain (amino acids 441-543) polyelectrolyte - 0.3 mg/ml - 20 mM Tris pH 8.0, 250 mM NaCl

Neurofilament light polypeptide (T445N; C-terminus, amino acids 441-543)
MWexperimental 20 kDa
MWexpected 12 kDa
log I(s) 2.77×10-2 2.77×10-3 2.77×10-4 2.77×10-5
Neurofilament light polypeptide (T445N; C-terminus, amino acids 441-543) small angle scattering data  s, nm-1
ln I(s)
Neurofilament light polypeptide (T445N; C-terminus, amino acids 441-543) Guinier plot ln 2.77×10-2 Rg: 4.4 nm 0 (4.4 nm)-2 s2
(sRg)2I(s)/I(0)
Neurofilament light polypeptide (T445N; C-terminus, amino acids 441-543) Kratky plot 1.104 0 √3 sRg

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of truncated neurofilament low intrinsically disordered tail domain in 20 mM Tris pH 8.00, 250 mM NaCl were collected on the EMBL P12 beam line at the PETRA III storage ring (DESY; Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.123982 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 0.30 mg/ml was measured at 24°C. 35 successive 0.095 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Data are on an absolute scale and normalised to protein concentration.

Tags: idp
Neurofilament light polypeptide (T445N; C-terminus, amino acids 441-543)
Mol. type   Protein
Organism   Mus musculus
Olig. state   Monomer
Mon. MW   12.2 kDa
 
UniProt   P08551 (441-543)
Sequence   FASTA