Beyond the VSG Layer: Exploring the Role of Intrinsic Disorder in the Invariant Surface Glycoproteins of African Trypanosomes.

Sülzen H, Volkov AN, Geens R, Zahedifard F, Stijlemans B, Zoltner M, Magez S, Sterckx YG, Zoll S, PLoS Pathog 20(4):e1012186 (2024) Europe PMC

SASDTB5 – Trypanosoma brucei gambiense variant surface glycoprotein 3.1

Variant surface glycoprotein 3.1
MWexperimental 102 kDa
MWexpected 103 kDa
VPorod 149 nm3
log I(s) 1.29×10-1 1.29×10-2 1.29×10-3 1.29×10-4
Variant surface glycoprotein 3.1 small angle scattering data  s, nm-1
ln I(s)
Variant surface glycoprotein 3.1 Guinier plot ln 1.30×10-1 Rg: 4.7 nm 0 (4.7 nm)-2 s2
(sRg)2I(s)/I(0)
Variant surface glycoprotein 3.1 Kratky plot 1.104 0 √3 sRg
p(r)
Variant surface glycoprotein 3.1 pair distance distribution function Rg: 4.8 nm 0 Dmax: 17.0 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Variant surface glycoprotein 3.1 BILBOMD model
Variant surface glycoprotein 3.1 BILBOMD model
Variant surface glycoprotein 3.1 BILBOMD model
Synchrotron SAXS data from solutions of Trypanosoma brucei gambiense variant surface glycoprotein 3.1 in 20 mM HEPES, 150 mM NaCl, 3% glycerol, pH 7.5 were collected on the SWING beam line at the SOLEIL storage ring (Saint-Aubin, France) using a Eiger 4M detector at a sample-detector distance of 2 m and at a wavelength of λ = 0.099 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 50.00 μl sample at 9.9 mg/ml was injected at a 0.20 ml/min flow rate onto a Shodex KW404-4F column at 20°C. 1000 successive 0.750 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

For display purposes the SAXS intensities have been scaled to the model-fit data (I(s) multiplied by 1000000).

Variant surface glycoprotein 3.1 (TbgVSG3.1)
Mol. type   Protein
Organism   Trypanosoma brucei gambiense
Olig. state   Dimer
Mon. MW   51.6 kDa
 
UniProt   M4N7P1 (1-482)
Sequence   FASTA