Synchrotron SAXS
data from solutions of
UP1 domain of heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) at 250 MPa
in
100 mM HEPES, 350 mM NaCl, 0.5 mM EDTA, pH 7.5
were collected
on the
G1 beam line
at the Cornell High Energy Synchrotron Source (CHESS) storage ring
(Ithaca, NY, USA)
using a Eiger 4M detector
at a sample-detector distance of 1.8 m and
at a wavelength of λ = 0.088 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
Solute concentrations ranging between 10 and 10 mg/ml were measured
at 17°C.
10 successive
1 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
s, nm-1
