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Synchrotron SAXS data from solutions of human CELSR1 EC4-7 in 20 mM Tris-HCl, 150 mM KCl, 50 mM NaCl, 2 mM CaCl2, 1 mM TCEP, pH 8 were collected on the 12.3.1 (SIBYLS) beam line at the Advanced Light Source (ALS; Berkeley, CA, USA) using a MAR 165 CCD detector at a sample-detector distance of 2.1 m and at a wavelength of λ = 0.1127 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A sample at 8 mg/ml was injected at a 0.65 ml/min flow rate onto a Shodex LW-803 column at 25°C. 660 successive 2 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
Sample injection volume = UNKNOWN. CAUTION. The dummy atom models shown in this entry show: 1) - Top - The volume and bead-occupancy corrected dummy-atom shape derived from a spatially aligned cohort of individual model reconstructions (DAMFILT) and; 2) - Bottom - A representative individual model and model-fit to the data.
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s, nm-1
