Synchrotron SAXS data from solutions of the LCP domain of LytR with geranylgeranyl diphosphate in 50 mM HEPES, 150 mM NaCl, 5 mM MgCl2, pH 8 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 3 2M detector at a wavelength of λ = 0.1 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 2.2 and 0.1 mg/ml were measured at 20°C. 10 successive frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.
s, nm-1
