Synchrotron SAXS
data from solutions of
Tissue Transglutaminase + CaCl2 + inhibitor 5826 + GTP: Equilibrium
in
20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, 2 mM CaCl2, 50 µM 5826, 5 mM GTP, pH 7.5
were collected
on the
ID7A1 BioSAXS / HP-Bio Beamline beam line
at the Cornell High Energy Synchrotron Source (CHESS) storage ring
(Ithaca, NY, USA)
using a Eiger 4M detector
at a sample-detector distance of 1.6 m and
at a wavelength of λ = 0.123 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
One solute concentration of 2.00 mg/ml was measured.
15 successive
5 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
TG2 was incubated with 2 mM CaCl2 for 5 minutes. Next, 50 µM of 5826 inhibitor were added, and the system was incubated for 30 minutes. Finally, 5 mM GTP was spiked in before measurement. Experimental temperature = UNKNOWN.
s, nm-1
