The conformational space of RNase P RNA in solution

Lee Y, Degenhardt M, Skeparnias I, Degenhardt H, Bhandari Y, Yu P, Stagno J, Fan L, Zhang J, Wang Y, Nature (2024) DOI

SASDTG7 – RNase P RNA in the presence of 5.0 mM MgCl2

RNase P RNA
MWexperimental 137 kDa
MWexpected 135 kDa
VPorod 277 nm3
log I(s) 2.10×101 2.10×100 2.10×10-1 2.10×10-2
RNase P RNA small angle scattering data  s, nm-1
ln I(s)
RNase P RNA Guinier plot ln 2.10×101 Rg: 4.7 nm 0 (4.7 nm)-2 s2
(sRg)2I(s)/I(0)
RNase P RNA Kratky plot 1.104 0 √3 sRg
p(r)
RNase P RNA pair distance distribution function Rg: 4.7 nm 0 Dmax: 14.6 nm

Data validation

There are no models related to this curve.

SAXS data from solutions of RNase P RNA in 100 mM NaCl, 5 mM MgCl2, pH 7.5 were collected using a Rigaku BIOSAXS-2000 instrument at the Center for Cancer Research (National Cancer Institute, Frederick MD USA) equipped with a Rigaku/P100K detector at a sample-detector distance of 0.5 m and at a wavelength of λ = 0.154 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 0.54 mg/ml was measured at 10°C. Eight successive 900 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

The sample was freshly prepared and the data collected just after SEC separation.

RNase P RNA (RNase P)
Mol. type   RNA
Organism   Geobacillus stearothermophilus
Olig. state   Monomer
Mon. MW   135.4 kDa
Sequence   FASTA