Structural insights of an LCP protein-LytR-from Streptococcus dysgalactiae subs. dysgalactiae through biophysical and in silico methods.

Paquete-Ferreira J, Freire F, Fernandes HS, Muthukumaran J, Ramos J, Bryton J, Panjkovich A, Svergun D, Santos MFA, Correia MAS, Fernandes AR, Romão MJ, Sousa SF, Santos-Silva T, Front Chem 12:1379914 (2024) Europe PMC

SASDTH2 – LCP domain of LytR from Streptococcus dysgalactiae in the absence of ligand (150 mM NaCl)

Biofilm regulatory protein
MWexperimental 32 kDa
MWexpected 34 kDa
VPorod 76 nm3
log I(s) 9.18×101 9.18×100 9.18×10-1 9.18×10-2
Biofilm regulatory protein small angle scattering data  s, nm-1
ln I(s)
Biofilm regulatory protein Guinier plot ln 9.18×101 Rg: 2.4 nm 0 (2.4 nm)-2 s2
(sRg)2I(s)/I(0)
Biofilm regulatory protein Kratky plot 1.104 0 √3 sRg
p(r)
Biofilm regulatory protein pair distance distribution function Rg: 2.4 nm 0 Dmax: 9 nm

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of the LCP domain of LytR in 50 mM HEPES, 150 mM NaCl, 5 mM MgCl2, pH 8 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 3 2M detector at a wavelength of λ = 0.1 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 3.1 and 0.2 mg/ml were measured at 20°C. 10 successive frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.

Sample detector distance = UNKNOWN. X-ray Exposure time = UNKNOWN

Biofilm regulatory protein (LytR/BrpA)
Mol. type   Protein
Organism   Streptococcus dysgalactiae subsp. dysgalactiae
Olig. state   Monomer
Mon. MW   33.6 kDa
 
UniProt   A0A0A7LTX5 (48-342)
Sequence   FASTA