From isolated polyelectrolytes to star-like assemblies: the role of sequence heterogeneity on the statistical structure of the intrinsically disordered neurofilament-low tail domain.

Kravikass M, Koren G, Saleh OA, Beck R, Eur Phys J E Soft Matter 47(2):13 (2024) Europe PMC

SASDTM6 – Neurofilament low intrinsically disordered tail domain aggregate - 0.6 mg/ml - 20 mM Tris pH 8.0, 350 mM NaCl

Neurofilament light polypeptide (T445N; C-terminus, amino acids 399-553)
MWexperimental 87 kDa
MWexpected 16 kDa
log I(s) 6.14×10-1 6.14×10-2 6.14×10-3 6.14×10-4
Neurofilament light polypeptide (T445N; C-terminus, amino acids 399-553) small angle scattering data  s, nm-1
ln I(s)
Neurofilament light polypeptide (T445N; C-terminus, amino acids 399-553) Guinier plot ln 6.15×10-1 Rg: 22.4 nm 0 (22.4 nm)-2 s2
(sRg)2I(s)/I(0)
Neurofilament light polypeptide (T445N; C-terminus, amino acids 399-553) Kratky plot 1.104 0 √3 sRg

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of neurofilament low intrinsically disordered tail domain aggregate in 20 mM Tris pH 8.00, 350 mM NaCl were collected on the EMBL P12 beam line at the PETRA III storage ring (DESY; Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.123982 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 0.60 mg/ml was measured at 24°C. 35 successive 0.095 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Neurofilament low intrinsically disordered tail domain with an aggregation number of 5.33 and radius of gyration of 9.81 nm as obtained by the fitting procedure described in the paper. Data are on an absolute scale and normalised to protein concentration.

Tags: idp
Neurofilament light polypeptide (T445N; C-terminus, amino acids 399-553) (NFLt)
Mol. type   Protein
Organism   Mus musculus
Olig. state   Unknown
Mon. MW   16.2 kDa
 
UniProt   P08551 (399-553)
Sequence   FASTA