Mechanisms of transcription attenuation and condensation of RNA polymerase II by RECQ5

Tomas Klumpler.

SASDTP7 – RECQ like helicase 5 fragment (amino acids 625-825; 0.6 mg/ml)

ATP-dependent DNA helicase Q5
MWexperimental 57 kDa
MWexpected 109 kDa
VPorod 116 nm3
log I(s) 7.04×10-1 7.04×10-2 7.04×10-3 7.04×10-4
ATP-dependent DNA helicase Q5 small angle scattering data  s, nm-1
ln I(s)
ATP-dependent DNA helicase Q5 Guinier plot ln 7.05×10-1 Rg: 4.3 nm 0 (4.3 nm)-2 s2
(sRg)2I(s)/I(0)
ATP-dependent DNA helicase Q5 Kratky plot 1.104 0 √3 sRg
p(r)
ATP-dependent DNA helicase Q5 pair distance distribution function Rg: 3.9 nm 0 Dmax: 11.2 nm

Data validation

There are no models related to this curve.

SAXS data from solutions of RECQ like helicase 5 fragment in 25 mM HEPES, 200 mM NaCl, 1 mM DTT, pH 7.5 were collected using a Rigaku BioSAXS-1000 instrument at CEITEC (Brno, Czech Republic) equipped with a Rigaku HyPix-3000 detector at a sample-detector distance of 0.5 m and at a wavelength of λ = 0.154 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 0.60 mg/ml was measured at 20°C. One 3600 second frame was collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Dmax appears underestimated.

ATP-dependent DNA helicase Q5 (RECQL5)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Unknown
Mon. MW   108.9 kDa
 
UniProt   O94762 (1-991)
Sequence   FASTA