From isolated polyelectrolytes to star-like assemblies: the role of sequence heterogeneity on the statistical structure of the intrinsically disordered neurofilament-low tail domain.

Kravikass M, Koren G, Saleh OA, Beck R, Eur Phys J E Soft Matter 47(2):13 (2024) Europe PMC

SASDTT6 – Truncated Neurofilament low intrinsically disordered tail domain (amino acids 441-543) polyelectrolyte - 0.8 mg/ml - 20 mM Tris pH 8.0

Neurofilament light polypeptide (T445N; C-terminus, amino acids 441-543)
MWexperimental 20 kDa
MWexpected 12 kDa
log I(s) 2.54×10-2 2.54×10-3 2.54×10-4 2.54×10-5
Neurofilament light polypeptide (T445N; C-terminus, amino acids 441-543) small angle scattering data  s, nm-1
ln I(s)
Neurofilament light polypeptide (T445N; C-terminus, amino acids 441-543) Guinier plot ln 2.54×10-2 Rg: 4.4 nm 0 (4.4 nm)-2 s2
(sRg)2I(s)/I(0)
Neurofilament light polypeptide (T445N; C-terminus, amino acids 441-543) Kratky plot 1.104 0 √3 sRg

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of truncated neurofilament low intrinsically disordered tail domain in 20 mM Tris, pH 8 were collected on the EMBL P12 beam line at the PETRA III storage ring (DESY; Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.123982 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 0.80 mg/ml was measured at 24°C. 35 successive 0.095 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Data are on an absolute scale and normalised to protein concentration.

Tags: idp
Neurofilament light polypeptide (T445N; C-terminus, amino acids 441-543)
Mol. type   Protein
Organism   Mus musculus
Olig. state   Monomer
Mon. MW   12.2 kDa
 
UniProt   P08551 (441-543)
Sequence   FASTA