| MWexperimental | 60 | kDa |
| MWexpected | 55 | kDa |
| VPorod | 102 | nm3 |
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log I(s)
4.72×103
4.72×102
4.72×101
4.72×100
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s, nm-1
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The Chlamydia pneumoniae effector SemD exploits its host’s endocytic machinery by structural and functional mimicry
Kocher F, Applegate V, Reiners J, Port A, Spona D, Hänsch S, Mirzaiebadizi A, Ahmadian M, Smits S, Hegemann J, Mölleken K, Nature Communications 15(1) (2024) DOISASDTU5 – SemDΔAPH bound to BR-GBD (actin nucleation-promoting factor WASL)
Uncharacterized proteinActin nucleation-promoting factor WASL
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Fits and models
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Rg, nm
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Synchrotron SAXS data from solutions of SemDΔAPH bound to BR-GBD in 137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, 3% glycerol, pH 8.5 were collected on the EMBL P12 beam line at PETRA III (DESY; Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 100.00 μl sample at 8 mg/ml was injected at a 0.60 ml/min flow rate onto a GE Superdex 200 Increase 10/300 column at 20°C. 2400 successive 0.995 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
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