Wu CG,
Balakrishnan VK,
Merrill RA,
Parihar PS,
Konovolov K,
Chen YC,
Xu Z,
Wei H,
Sundaresan R,
Cui Q,
Wadzinski BE,
Swingle MR,
Musiyenko A,
Chung WK,
Honkanen RE,
Suzuki A,
Huang X,
Strack S,
Xing Y,
Proc Natl Acad Sci U S A
121(1):e2310727120
(2024)
Europe PMC
SASDTW2 – Protein phosphatase 2A (PP2A)-B56δ holoenzyme wildtype
Synchrotron SAXS data from solutions of PP2A-B56δ holoenzyme wildtype in a buffer (25 mM Tris-HCl pH 8.0, 150 mM NaCl, 50 µM MnCl2) were collected on the BioCAT 18-ID-D beamline at the Advanced Photon Source (APS) (Chicago, IL, USA) using a Pilatus3 X 1M detector (Dectris) at a sample-detector distance of ~3.7 m and at a wavelength of λ = 0.1033 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC)-SAXS was employed. The SEC parameters were as follows: A 550 μl sample at 1.5 mg/ml was injected at a 0.70 ml/min flow rate onto a Superdex 200 10/300 column (GE healthcare) at 22°C. 2000 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
s, nm-1
