Molecular insights into the structure and function of the Staphylococcus aureus fatty acid kinase.

Myers MJ, Xu Z, Ryan BJ, DeMars ZR, Ridder MJ, Johnson DK, Krute CN, Flynn TS, Kashipathy MM, Battaile KP, Schnicker N, Lovell S, Freudenthal BD, Bose JL, J Biol Chem 300(12):107920 (2024) Europe PMC

SASDTW8 – Staphylococcus aureus Fatty Acid Kinase A

Uncharacterized protein SAUSA300_1119
MWexperimental 129 kDa
MWexpected 125 kDa
VPorod 219 nm3
log I(s) 2.90×100 2.90×10-1 2.90×10-2 2.90×10-3
Uncharacterized protein SAUSA300_1119 small angle scattering data  s, nm-1
ln I(s)
Uncharacterized protein SAUSA300_1119 Guinier plot ln 2.90×100 Rg: 4.3 nm 0 (4.3 nm)-2 s2
(sRg)2I(s)/I(0)
Uncharacterized protein SAUSA300_1119 Kratky plot 1.104 0 √3 sRg
p(r)
Uncharacterized protein SAUSA300_1119 pair distance distribution function Rg: 4.3 nm 0 Dmax: 16 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Uncharacterized protein SAUSA300_1119 DAMMIF model
log I(s)
 s, nm-1
Uncharacterized protein SAUSA300_1119 DAMMIF model
log I(s)
 s, nm-1
Synchrotron SAXS data from solutions of Fatty acid kinase A (FakA) in 50 mM Tris-HCl pH 7.4, 150 mM KCl, 5% glycerol, 1 mM TCEP were collected on the BioCAT 18-ID-D beamline at the Advanced Photon Source (APS), Argonne National Laboratory storage ring (Lemont, IL, USA) using a Pilatus3 X 1M detector at a sample-detector distance of 3.663 m and at a wavelength of λ = 0.1033 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC)-SAXS was employed. The SEC parameters were as follows: A 200 μl sample at 4.7 mg/ml was injected at a 0.60 ml/min flow rate onto a Superdex 200 Increase GL 10/300 column (GE healthcare) at 23°C. 2400 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Uncharacterized protein SAUSA300_1119 (FakA)
Mol. type   Protein
Organism   Staphylococcus aureus (strain USA300)
Olig. state   Dimer
Mon. MW   62.7 kDa
 
UniProt   Q2FHL2 (1-548)
Sequence   FASTA