Synchrotron SAXS
data from solutions of
Heterogeneous nuclear ribonucleoprotein A1-A (hnRNP A1-A; SEC-SAXS, Sept 2022)
in
100 mM HEPES, 350 mM NaCl, 0.5 mM EDTA, pH 7.5
were collected
on the
12-ID-B beam line
at the Advanced Photon Source (APS), Argonne National Laboratory storage ring
(Lemont, IL, USA)
using a Eiger2 S detector
at a sample-detector distance of 3.7 m and
at a wavelength of λ = 0.103 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 200.00 μl sample
at 3.5 mg/ml was injected at a 0.60 ml/min flow rate
onto a Cytiva Superdex 200 Increase 10/300 column
at 22°C.
2457 successive
0.500 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
s, nm-1
