| MWexperimental | 128 | kDa |
| MWexpected | 127 | kDa |
| VPorod | 327 | nm3 |
|
log I(s)
1.60×10-2
1.60×10-3
1.60×10-4
1.60×10-5
|
s, nm-1
|
Autoinhibition of ubiquitin-specific protease 8: insights into domain interactions and mechanisms of regulation
Caba C, Black M, Liu Y, DaDalt A, Mallare J, Fan L, Harding R, Wang Y, Vacratsis P, Huang R, Zhuang Z, Tong Y, Journal of Biological Chemistry :107727 (2024) DOISASDU39 – Nearly full-length monomeric Human Ubiquitin carboxyl-terminal hydrolase 8 (USP8) (7-1110)
Ubiquitin carboxyl-terminal hydrolase 8|
|
|
Fits and models
|
|
|
Synchrotron SAXS
data from solutions of
Nearly full-length monomeric Human Ubiquitin carboxyl-terminal hydrolase 8 (USP8) (7-1110)
in
25 mM Tris, 150 mM NaCl, pH 7.5
were collected
on the
BioCAT 18ID beam line
at the Advanced Photon Source (APS), Argonne National Laboratory storage ring
(Lemont, IL, USA)
using a Eiger2 XE 9M detector
at a sample-detector distance of 3.6 m and
at a wavelength of λ = 0.1033 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 350.00 μl sample
at 2 mg/ml was injected at a 0.60 ml/min flow rate
onto a GE Superdex 200 Increase 10/300 column
at 22°C.
Nine successive
0.500 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
|
|
|||||||||||||||||||||||||||