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Synchrotron SAXS data from solutions of the Z-DNA binding domains Zαβ of Z-DNA binding protein 1 in 20 mM Tris, 100 mM NaCl, pH 7.5 were collected on the BL19U2 beam line at the Shanghai Synchrotron Radiation Facility (SSRF; Shanghai, China) using a Pilatus 1M detector at a wavelength of λ = 0.103 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 1.00 mg/ml was measured at 20°C. 20 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
The models displayed in this entry show an individual DAMMIF ab initio shape reconstruction (top) and the associated individual model fit to the data, in addition to the disposition of the protein based on the spatial alignment of 10 individual models (volume and bead-occupancy corrected - DAMFILT model, bottom). The 10 individual models that were used to generate the DAMFILT model are provided in the full entry zip archive. Sample detector distance = UNKNOWN.
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s, nm-1
