| MWexperimental | 80 | kDa |
| MWexpected | 83 | kDa |
| VPorod | 127 | nm3 |
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log I(s)
1.19×10-1
1.19×10-2
1.19×10-3
1.19×10-4
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s, nm-1
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Integrative structural analysis of NF45-NF90 heterodimers reveals architectural rearrangements and oligomerization on binding dsRNA.
Winterbourne S, Jayachandran U, Zou J, Rappsilber J, Granneman S, Cook AG, Nucleic Acids Res 53(6) (2025) Europe PMCSASDUB5 – Heterodimer complex of domain-associated zinc finger domains of Interleukin enhancer-binding factor 2 (29-390) and Interleukin enhancer-binding factor 3 (1-381)
Interleukin enhancer-binding factor 3Interleukin enhancer-binding factor 2
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Fits and models
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Synchrotron SAXS
data from solutions of
Heterodimer complex of domain-associated zinc finger domains of Interleukin enhancer-binding factor 2 (29-390) and Interleukin enhancer-binding factor 3 (1-381)
in
20 mM HEPES, 150 mM NaCl, 1 mM DTT, pH 7.5
were collected
on the
B21 beam line
at the Diamond Light Source storage ring
(Didcot, UK)
using a Eiger 4M detector
at a sample-detector distance of 3.7 m and
at a wavelength of λ = 0.0954 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 60.00 μl sample
at 10 mg/ml was injected at a 0.10 ml/min flow rate
onto a Cytiva Superdex 200 Increase 3.2/300 column
at 15°C.
915 successive
0.005 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
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