Intramolecular autoinhibition regulates the selectivity of PRPF40A tandem WW domains for proline-rich motifs.

Martínez-Lumbreras S, Träger LK, Mulorz MM, Payr M, Dikaya V, Hipp C, König J, Sattler M, Nat Commun 15(1):3888 (2024) Europe PMC

SASDUH4 – Tandem of WW domains of Pre-mRNA-processing factor 40 homolog A (PRPF40A) in HEPES buffer

Pre-mRNA-processing factor 40 homolog A
MWexperimental 10 kDa
MWexpected 11 kDa
VPorod 18 nm3
log I(s) 9.69×10-2 9.69×10-3 9.69×10-4 9.69×10-5
Pre-mRNA-processing factor 40 homolog A small angle scattering data  s, nm-1
ln I(s)
Pre-mRNA-processing factor 40 homolog A Guinier plot ln 9.70×10-2 Rg: 2.0 nm 0 (2.0 nm)-2 s2
(sRg)2I(s)/I(0)
Pre-mRNA-processing factor 40 homolog A Kratky plot 1.104 0 3 sRg
p(r)
Pre-mRNA-processing factor 40 homolog A pair distance distribution function Rg: 2.0 nm 0 Dmax: 6.3 nm

Data validation


There are no models related to this curve.

SAXS data from solutions of the tandem WW domains of PRPF40A in 20 mM HEPES, 100 mM NaCl, 1 mM DTT, pH 6.6 were collected using a Rigaku BioSAXS-1000 instrument (Technische Universität München, Garching, Germany) equipped with a Rigaku / HyPix-3000 detector at a sample-detector distance of 0.5 m and at a wavelength of λ = 0.15406 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 1.00 mg/ml was measured at 25°C. Eight successive 900 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Pre-mRNA-processing factor 40 homolog A (PRPF40A)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   11.4 kDa
 
UniProt   O75400 (141-236)
Sequence   FASTA