Structural basis of the monkeypox virus mRNA cap N7 methyltransferase complex.

Chen A, Fang N, Zhang Z, Wen Y, Shen Y, Zhang Y, Zhang L, Zhao G, Ding J, Li J, Emerg Microbes Infect 13(1):2369193 (2024) Europe PMC

SASDUM2 – mRNA capping enzyme MTase

Virus termination factor small subunit
mRNA-capping enzyme catalytic subunit
MWexperimental 67 kDa
MWexpected 68 kDa
VPorod 106 nm3
log I(s) 5.31×101 5.31×100 5.31×10-1 5.31×10-2
Virus termination factor small subunit mRNA-capping enzyme catalytic subunit small angle scattering data  s, nm-1
ln I(s)
Virus termination factor small subunit mRNA-capping enzyme catalytic subunit Guinier plot ln 5.31×101 Rg: 2.9 nm 0 (2.9 nm)-2 s2
(sRg)2I(s)/I(0)
Virus termination factor small subunit mRNA-capping enzyme catalytic subunit Kratky plot 1.104 0 √3 sRg
p(r)
Virus termination factor small subunit mRNA-capping enzyme catalytic subunit pair distance distribution function Rg: 2.9 nm 0 Dmax: 10.1 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Virus termination factor small subunit mRNA-capping enzyme catalytic subunit DAMFILT model
Virus termination factor small subunit mRNA-capping enzyme catalytic subunit DAMMIF model
Synchrotron SAXS data from solutions of mRNA capping enzyme MTase in 20 mM Tris, 150 mM NaCl, pH 8 were collected on the BL19U2 beam line at the Shanghai Synchrotron Radiation Facility (SSRF; Shanghai, China) using a Pilatus 1M detector at a sample-detector distance of 1 m and at a wavelength of λ = 0.1033 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 1.00 mg/ml was measured at 25°C. 10 successive 6 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

The dummy atom models displayed in this entry show: Top - the minimised volume and bead-occupancy 'averaged' shape obtained from the spatial alignment of a ten-member individual model cohort (DAMFILT) and; Bottom - an example of an individual model, with the corresponding individual-model fit to the data (displayed to the left). The ten-member model cohort is available for download in the full entry zip archive.

Virus termination factor small subunit
Mol. type   Protein
Organism   Monkeypox virus (strain Zaire-96-I-16)
Olig. state   Monomer
Mon. MW   33 kDa
 
UniProt   Q8V4X6 (1-287)
Sequence   FASTA
 
mRNA-capping enzyme catalytic subunit
Mol. type   Protein
Organism   Monkeypox virus (strain Zaire-96-I-16)
Olig. state   Monomer
Mon. MW   34.6 kDa
 
UniProt   Q8V4Y7 (546-845)
Sequence   FASTA