Sugiyama A,
Minami M,
Ugajin K,
Inaba-Inoue S,
Yabuno N,
Takekawa Y,
Xiaomei S,
Takei S,
Sasaki M,
Nomai T,
Jiang X,
Kita S,
Maenaka K,
Hirose M,
Yao M,
Gooley P,
Moseley G,
Sugita Y,
Ose T,
Science Signaling
18(878)
(2025)
DOI
SASDUM4 – Signal transducer and activator of transcription 1-alpha/beta bound to phosphoprotein (STAT1/RVP)
Synchrotron SAXS data from solutions of STAT1/RVP in 10 mM HEPES-NaOH, 150 mM NaCl, 3% glycerol, 2 mM DTT, pH 7.4 were collected on the BL-10C beam line at the Photon Factory High Energy Accelerator Research Organization (KEK; Tsukuba, Japan) using a Pilatus3 2M detector at a sample-detector distance of 2.1 m and at a wavelength of λ = 0.1 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 130.00 μl sample at 9.2 mg/ml was injected at a 0.05 ml/min flow rate onto a GE Superdex 200 Increase 10/300 column at 25°C. 279 successive 20 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
s, nm-1
