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SAXS
data from solutions of
Mycobacterium smegmatis Inosine-5'-monophosphate dehydrogenase (IMPDH) - IMP-bound form
in
50 mM HEPES, 200 mM KCl, 2 mM MgCl2, 0.5 mM TCEP, pH 7.5
were collected
using a Eiger R 1M detector
at a sample-detector distance of 0.8 m and
at a wavelength of λ = 0.134 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
One solute concentration of 3.00 mg/ml was measured
at 20°C.
60 successive
30.580 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
SAXS data from solutions of IMPDH - IMP-bound form - in 50 mM HEPES, 200 mM KCl, 2 mM MgCl2, 0.5 mM TCEP, pH 7.5 were collected using an Anton Paar SAXSpoint 2.0 instrument at the Institute of Biotechnology, Czech Academy of Sciences (Centre of Molecular Structure, Vestec, Czech Republic) equipped with an Eiger R 1M detector at a sample-detector distance of 0.8 m and at a wavelength of λ = 0.134 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 3.00 mg/ml was measured at 20°C. 60 successive 30.580 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
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s, nm-1
