Photocobilins integrate B(12) and bilin photochemistry for enzyme control.

Zhang S, Jeffreys LN, Poddar H, Yu Y, Liu C, Patel K, Johannissen LO, Zhu L, Cliff MJ, Yan C, Schirò G, Weik M, Sakuma M, Levy CW, Leys D, Heyes DJ, Scrutton NS, Nat Commun 15(1):2740 (2024) Europe PMC

SASDUS3 – Dark-adapted photocobilin diguanylate cyclase fusion from Acidimicrobiaceae bacterium

GGDEF domain-containing protein
MWexperimental 130 kDa
MWexpected 132 kDa
VPorod 194 nm3
log I(s) 1.01×103 1.01×102 1.01×101 1.01×100
GGDEF domain-containing protein small angle scattering data  s, nm-1
ln I(s)
GGDEF domain-containing protein Guinier plot ln 1.02×103 Rg: 4.1 nm 0 (4.1 nm)-2 s2
(sRg)2I(s)/I(0)
GGDEF domain-containing protein Kratky plot 1.104 0 √3 sRg
p(r)
GGDEF domain-containing protein pair distance distribution function Rg: 4.2 nm 0 Dmax: 14.9 nm

Data validation

Fits and models

log I(s)
 s, nm-1
GGDEF domain-containing protein DAMMIF model
Synchrotron SAXS data from solutions of a dark-adapted photocobilin diguanylate cyclase fusion in 20 mM HEPES, 150 mM NaCl, pH 6.8 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus3 2M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.099187 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 5.00 mg/ml was measured at 20°C. 10 successive 0.990 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

GGDEF domain-containing protein
Mol. type   Protein
Organism   Acidimicrobiaceae bacterium
Olig. state   Dimer
Mon. MW   66.1 kDa
 
UniProt   A0A349B205 (1-608)
Sequence   FASTA