| MWexperimental | 69 | kDa |
| MWexpected | 110 | kDa |
| VPorod | 86 | nm3 |
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log I(s)
4.87×102
4.87×101
4.87×100
4.87×10-1
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s, nm-1
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A Simple Protocol for Visualization of RNA-Protein Complexes by Atomic Force Microscopy.
Tripepi A, Shakoor H, Klapetek P, Curr Protoc 5(1):e70084 (2025) Europe PMCSASDUS5 – Human Double-stranded RNA-binding protein Staufen homolog 1
Double-stranded RNA-binding protein Staufen homolog 1 (Δ1-81)|
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Fits and models
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Rg, nm
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Synchrotron SAXS data from solutions of double-stranded RNA-binding protein staufen homolog 1 in 50 mM TRIS, 300 mM NaCl, 3.8 mM β-mercaptoethanol, pH 7 were collected on the EMBL P12 beam line at PETRA III (DESY; Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 100.00 μl sample at 5.9 mg/ml was injected at a 0.60 ml/min flow rate onto a GE Superdex 200 Increase 10/300 column at 20°C. One 1 second frame was collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
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