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Synchrotron SAXS data from solutions of recombinant short complement regulator SCR-17/18H in 10 mM HEPES, 137 mM NaCl, pH 7.4 were collected on the B21 beam line at the Diamond Light Source (Didcot, UK) using a Eiger 4M detector at a sample-detector distance of 4 m and at a wavelength of λ = 0.12 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 1.50 mg/ml was measured at 20°C. During batch mode operations, an automatic sampler (96-well plate) transferred 35 µl of the sample from the plate to a temperature-controlled quartz capillary cell with a 1.5 mm diameter. Data were recorded in 30-frame sets, each frame having an exposure time of 1 second, and each set was acquired in duplicate to confirm reproducibility. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
Batch mode data were collected across several sessions between 2019 and 2021 using the SCR-17/18H with PNGase F treatment (recombinant glycosidase) to remove N-linked glycosylation. Protein produced in Pichia pastoris (Komagataella pastoris). A non-standard fit file is displayed (comparison between interpolated experimental data and theoretical fitting data).
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s, nm-1
