A Simple Protocol for Visualization of RNA-Protein Complexes by Atomic Force Microscopy.

Tripepi A, Shakoor H, Klapetek P, Curr Protoc 5(1):e70084 (2025) Europe PMC

SASDUT5 – Human Double-stranded RNA-binding protein Staufen homolog 1 with truncated Staufen-swapping motif (ΔSSM) monomeric structures

Double-stranded RNA-binding protein Staufen homolog 1 with truncated Staufen-swapping motif (ΔSSM)
MWexperimental 11 kDa
MWexpected 52 kDa
VPorod 51 nm3
log I(s) 3.82×10-1 3.82×10-2 3.82×10-3 3.82×10-4
Double-stranded RNA-binding protein Staufen homolog 1 with truncated Staufen-swapping motif (ΔSSM) small angle scattering data  s, nm-1
ln I(s)
Double-stranded RNA-binding protein Staufen homolog 1 with truncated Staufen-swapping motif (ΔSSM) Guinier plot ln 3.82×10-1 Rg: 3.8 nm 0 (3.8 nm)-2 s2
(sRg)2I(s)/I(0)
Double-stranded RNA-binding protein Staufen homolog 1 with truncated Staufen-swapping motif (ΔSSM) Kratky plot 1.104 0 √3 sRg
p(r)
Double-stranded RNA-binding protein Staufen homolog 1 with truncated Staufen-swapping motif (ΔSSM) pair distance distribution function Rg: 4.2 nm 0 Dmax: 15.1 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Human Double-stranded RNA-binding protein Staufen homolog 1 with truncated Staufen-swapping motif (ΔSSM) monomeric structures Rg histogram Rg, nm
Double-stranded RNA-binding protein Staufen homolog 1 with truncated Staufen-swapping motif (ΔSSM) EOM/RANCH model
Double-stranded RNA-binding protein Staufen homolog 1 with truncated Staufen-swapping motif (ΔSSM) EOM/RANCH model
Double-stranded RNA-binding protein Staufen homolog 1 with truncated Staufen-swapping motif (ΔSSM) EOM/RANCH model
Double-stranded RNA-binding protein Staufen homolog 1 with truncated Staufen-swapping motif (ΔSSM) EOM/RANCH model
SAXS data from solutions of double-stranded RNA-binding protein Staufen homolog 1 with truncated Staufen-swapping motif (ΔSSM) in 50 mM TRIS, 300 mM NaCl, 3.8 mM β-mercaptoethanol, pH 7 were collected using a Rigaku BioSAXS-1000 instrument at CEITEC (Brno, Czech Republic) equipped with a Pilatus 100K detector at a sample-detector distance of 0.5 m and at a wavelength of λ = 0.154 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 1.50 mg/ml was measured at 20°C. One 3600 second frame was collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Double-stranded RNA-binding protein Staufen homolog 1 with truncated Staufen-swapping motif (ΔSSM) (Stau1 ΔSSM)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   51.5 kDa
Sequence   FASTA