| MWexperimental | 94 | kDa |
| MWexpected | 103 | kDa |
| VPorod | 129 | nm3 |
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log I(s)
7.98×10-1
7.98×10-2
7.98×10-3
7.98×10-4
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s, nm-1
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A Simple Protocol for Visualization of RNA-Protein Complexes by Atomic Force Microscopy.
Tripepi A, Shakoor H, Klapetek P, Curr Protoc 5(1):e70084 (2025) Europe PMCSASDUU5 – Human Double-stranded RNA-binding protein Staufen homolog 1 with truncated RNA-binding domain 2 bound to 3'UTR fragment of ADP-ribosylation factor 1
Double-stranded RNA-binding protein Staufen homolog 1 (Δ1-177)3'UTR fragment of ADP-ribosylation factor 1
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Fits and models
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SAXS data from solutions of double-stranded RNA-binding protein Staufen homolog 1 with truncated RNA-binding domain 2 bound to the 3'UTR fragment of ADP-ribosylation factor 1 in 50 mM TRIS, 300 mM NaCl, 3.8 mM β-mercaptoethanol, pH 7 were collected using a Rigaku BioSAXS-1000 instrument at CEITEC (Brno, Czech Republic) equipped with a Pilatus 100K detector at a sample-detector distance of 0.5 m and at a wavelength of λ = 0.154 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 4.00 mg/ml was measured at 4°C. One 3600 second frame was collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
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