Visualizing the nucleating and capped states of f-actin by Ca(2+)-gelsolin: Saxs data based structures of binary and ternary complexes.

Sagar A, Peddada N, Choudhary V, Mir Y, Garg R, Ashish, Int J Biol Macromol :134556 (2024) Europe PMC

SASDUW3 – Calcium activated full-length gelsolin

Gelsolin
MWI(0) 82 kDa
MWexpected 84 kDa
VPorod 129 nm3
log I(s) 5.37×103 5.37×102 5.37×101 5.37×100
Gelsolin small angle scattering data  s, nm-1
ln I(s)
Gelsolin Guinier plot ln 5.38×103 Rg: 4.2 nm 0 (4.2 nm)-2 s2
(sRg)2I(s)/I(0)
Gelsolin Kratky plot 1.104 0 √3 sRg
p(r)
Gelsolin pair distance distribution function Rg: 4.3 nm 0 Dmax: 16 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Gelsolin PYMOL model
log I(s)
 s, nm-1
Gelsolin SREFLEX model
log I(s)
 s, nm-1
Gelsolin SREFLEX model
Synchrotron SAXS data from solutions of Calcium activated full-length gelsolin in 25 mM Tris-HCl, pH8, 45 mM NaCl, 1 mM EGTA + 4 mM CaCl2, pH 8 were collected on the EMBL P12 beam line at the PETRA III storage ring (DESY; Hamburg, Germany) using a Pilatus 2M detector at a sample-detector distance of 3.1 m and at a wavelength of λ = 0.155 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 2.50 mg/ml was measured at 10°C. 20 successive 0.050 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Gelsolin (rhuGSN)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   84.3 kDa
 
UniProt   P06396 (25-782)
Sequence   FASTA