| MWI(0) | 700 | kDa |
| MWexpected | 751 | kDa |
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log I(s)
3.17×104
3.17×103
3.17×102
3.17×101
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s, nm-1
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Visualizing the nucleating and capped states of f-actin by Ca(2+)-gelsolin: Saxs data based structures of binary and ternary complexes.
Sagar A, Peddada N, Choudhary V, Mir Y, Garg R, Ashish, Int J Biol Macromol :134556 (2024) Europe PMCSASDUX3 – F-actin in F-buffer at an actin concentration of 2 mg/mL
Actin, cytoplasmic 1|
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Fits and models
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Synchrotron SAXS
data from solutions of
F-actin in F-buffer at an actin concentration of 2 mg/mL
in
50 mM KCl, 50 mM Tris-HCl, pH 8.0, 5 mM MgCl2, 1 mM ATP, 0.1% 2-mercaptoethanol, pH 8
were collected
on the
EMBL P12 beam line
at the PETRA III storage ring
(DESY; Hamburg, Germany)
using a Pilatus 2M detector
at a sample-detector distance of 3.1 m and
at a wavelength of λ = 0.155 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
One solute concentration of 2.00 mg/ml was measured
at 10°C.
20 successive
0.050 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
CAUTION: The calculated p(r) is categorised as a SUSPICIOUS solution. |
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