Aspergillus niger beta-glucosidase (bgl1)

Estella Yee.

SASDUX7 – Aspergillus niger beta-glucosidase (bgl1), partially deglycosylated with Endoglycosidase H

beta-glucosidase
MWexperimental 178 kDa
MWexpected 187 kDa
VPorod 253 nm3
log I(s) 3.94×101 3.94×100 3.94×10-1 3.94×10-2
beta-glucosidase small angle scattering data  s, nm-1
ln I(s)
beta-glucosidase Guinier plot ln 3.95×101 Rg: 4.2 nm 0 (4.2 nm)-2 s2
(sRg)2I(s)/I(0)
beta-glucosidase Kratky plot 1.104 0 √3 sRg
p(r)
beta-glucosidase pair distance distribution function Rg: 4.4 nm 0 Dmax: 14 nm

Data validation

Fits and models

log I(s)
 s, nm-1
beta-glucosidase GASBOR model
Synchrotron SAXS data from solutions of Aspergillus niger beta-glucosidase (bgl1) in 50 mM sodium acetate, pH 5 were collected on the 16-ID (LiX) beamline at the National Synchrotron Light Source II (NSLS-II) storage ring (Upton, NY, USA) using a Pilatus3 X 1M, Pilatus3 X 900K detector at a sample-detector distance of 3.6 m and at a wavelength of λ = 0.08189 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 99.00 μl sample at 4.5 mg/ml was injected at a 0.35 ml/min flow rate onto a Biozen 3 µm dSEC-2, 200 Å column. 10 successive 2-second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

For the experiment, SAXS and WAXS detectors were used simultaneously. The detector positions are Sample-to-detector distance (in meters): SAXS - 3.562, WAXS - 0.317

beta-glucosidase (bgl1)
Mol. type   Protein
Organism   Aspergillus niger
Olig. state   Dimer
Mon. MW   93.3 kDa
 
UniProt   Q9P8F4 (1-860)
Sequence   FASTA