Conformational signatures induced by ubiquitin modification in the amyloid-associated tau repeat domain

Gabriele Giachin.

SASDV46 – Microtubule-associated protein tau, lysine-rich four-repeat domain 4RD

Isoform Tau-F of Microtubule-associated protein tau (C291A, K311C, K317C, C322A)
MWexperimental 15 kDa
MWexpected 14 kDa
VPorod 33 nm3
log I(s) 6.78×10-2 6.78×10-3 6.78×10-4 6.78×10-5
Isoform Tau-F of Microtubule-associated protein tau (C291A, K311C, K317C, C322A) small angle scattering data  s, nm-1
ln I(s)
Isoform Tau-F of Microtubule-associated protein tau (C291A, K311C, K317C, C322A) Guinier plot ln 6.79×10-2 Rg: 3.5 nm 0 (3.5 nm)-2 s2
(sRg)2I(s)/I(0)
Isoform Tau-F of Microtubule-associated protein tau (C291A, K311C, K317C, C322A) Kratky plot 1.104 0 √3 sRg
p(r)
Isoform Tau-F of Microtubule-associated protein tau (C291A, K311C, K317C, C322A) pair distance distribution function Rg: 3.6 nm 0 Dmax: 12.2 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Microtubule-associated protein tau, lysine-rich four-repeat domain 4RD Rg histogram Rg, nm
Isoform Tau-F of Microtubule-associated protein tau (C291A, K311C, K317C, C322A) EOM/RANCH model
log I(s)
 s, nm-1
Isoform Tau-F of Microtubule-associated protein tau (C291A, K311C, K317C, C322A) AMBER model
Synchrotron SAXS data from solutions of microtubule-associated protein tau (lysine-rich four-repeat domain 4RD) in 100 mM NaCl, pH 6.8 were collected on the EMBL P12 beam line at PETRA III (DESY; Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.123972 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 10.00 mg/ml was measured at 20°C. 20 successive 0.050 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Isoform Tau-F of Microtubule-associated protein tau (C291A, K311C, K317C, C322A) (tau4RD domain)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   13.7 kDa
 
UniProt   P10636-8 (244-372)
Sequence   FASTA