Structural dynamics of protein-protein association involved in the light-induced transition of Avena sativa LOV2 protein.

Kim C, Yun SR, Lee SJ, Kim SO, Lee H, Choi J, Kim JG, Kim TW, You S, Kosheleva I, Noh T, Baek J, Ihee H, Nat Commun 15(1):6991 (2024) Europe PMC

SASDV55 – The dark-adapted AsLOV2 domain (Avena sativa, Oat)

non-specific serine/threonine protein kinase
MWexperimental 17 kDa
MWexpected 17 kDa
VPorod 21 nm3
log I(s) 1.27×101 1.27×100 1.27×10-1 1.27×10-2
non-specific serine/threonine protein kinase small angle scattering data  s, nm-1
ln I(s)
non-specific serine/threonine protein kinase Guinier plot ln 1.27×101 Rg: 1.8 nm 0 (1.8 nm)-2 s2
(sRg)2I(s)/I(0)
non-specific serine/threonine protein kinase Kratky plot 1.104 0 3 sRg
Dmax: 6.6 nm

Data validation


There are no models related to this curve.

Synchrotron SAXS data from solutions of dark-adapted AsLOV2 domain in 20 mM Tris, 200 mM NaCl, pH 7 were collected on the 4C beam line at the Pohang Accelerator Laboratory (Pohang, South Korea) using a Rayonix SX165 detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.0734 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 24.98 mg/ml was measured at 24°C. 50 successive 10 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

non-specific serine/threonine protein kinase
Mol. type   Protein
Organism   Avena sativa
Olig. state   Monomer
Mon. MW   16.6 kDa
 
UniProt   O49003 (404-546)
Sequence   FASTA