SAXS data for AsLOV2

Hyotcherl Ihee.

SASDV55 – The dark-adapted AsLOV2 domain (Avena sativa, Oat)

non-specific serine/threonine protein kinase
MWexperimental 17 kDa
MWexpected 17 kDa
VPorod 21 nm3
log I(s) 1.27×101 1.27×100 1.27×10-1 1.27×10-2
non-specific serine/threonine protein kinase small angle scattering data  s, nm-1
ln I(s)
non-specific serine/threonine protein kinase Guinier plot ln 1.27×101 Rg: 1.8 nm 0 (1.8 nm)-2 s2
(sRg)2I(s)/I(0)
non-specific serine/threonine protein kinase Kratky plot 1.104 0 3 sRg
Dmax: 6.6 nm

Data validation


There are no models related to this curve.

Synchrotron SAXS data from solutions of dark-adapted AsLOV2 domain in 20 mM Tris, 200 mM NaCl, pH 7 were collected on the 4C beam line at the Pohang Accelerator Laboratory (Pohang, South Korea) using a Rayonix SX165 detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.0734 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 24.98 mg/ml was measured at 24°C. 50 successive 10 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

non-specific serine/threonine protein kinase
Mol. type   Protein
Organism   Avena sativa
Olig. state   Monomer
Mon. MW   16.6 kDa
 
UniProt   O49003 (404-546)
Sequence   FASTA